Isolation of Actinomyces viscosus strain GA: characteristics of its cellular biological retardant(s)
نویسندگان
چکیده
منابع مشابه
Isolation of Actinomyces viscosus from two patients with clinical infections.
The isolation of Actinomyces viscosus from two patients is described. One was a case of multiple myeloma, the organism being found on blood culture; the other was a patient with a submandibular abscess. These are believed to be the first such isolations of A. viscosus in this country.
متن کاملIsolation of a neuraminidase gene from Actinomyces viscosus T14V.
A genomic library of Actinomyces viscosus T14V DNA in lambda gt11 was screened for expression of neuraminidase activities. Four recombinant clones were detected that gave blue fluorescence upon incubation with a fluorogenic substrate, 2'-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid. Of these, two were identical, and all of the neuraminidase-positive clones shared a common 3.4-kbp DNA ...
متن کاملSurface fibrils (fimbriae) of Actinomyces viscosus T14V.
Surface antigens of Actinomyces viscosus T14V were released from cell walls by digestion with lysozyme. These were separated by ion-exchange and gel filtration chromatography into fractions rich in carbohydrate or protein. The former contained a polysaccharide high in 6-deoxytalose, along with a peptide fragment from the cell wall. In the protein-rich fractions, material of high molecular weigh...
متن کاملMannose-contaminating agglutinin for Actinomyces viscosus and Actinomyces naeslundii.
Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...
متن کاملRegulation of invertase of Actinomyces viscosus.
The regulation of diethylaminoethyl-partially purified invertase (EC 3.2.1.26; beta-D-fructofuranoside fructohydrolase) from the 37,000 X g-soluble intracellular fluid of Actinomyces viscosus serotype 2 strain M-100 was studied. Glycolytic intermediates, mono-, di-, and triphosphate nucleotides, inorganic phosphate, and various divalent cations were tested for regulatory effects. Fructose-6-pho...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 1997
ISSN: 0266-8254,1472-765X
DOI: 10.1046/j.1472-765x.1997.00178.x